The sequencing project of A. flavus (NRRL 3357) was led by Gary Payne and Ralph Dean at North Carolina State University and funded by the Microbial Genome Sequencing Project-USDA National Research Initiative. Using the whole-genome random shotgun method, this yielded 5x coverage and 24 unassigned supercontigs.

Sequencing and first-pass automated annotation was carried out by the JCVI. This was further improved by EST data provided by USDA/ARS/SRRC (New Orleans). USDA/ARS/SRRC also provided funds for sequence closure and finishing. The final phase, manual refinement of gene annotation, was undertaken by North Carolina State University. A more detailed report can found on the A. flavus project website.